Hemorrhage is caused due to the destruction of structural components of basement membrane surrounding blood vessels and capillaries. Predominantly, zinc dependent metalloproteases of the ‘metzincin’ family enzymes of the venom are found responsible for this toxic property. Hemorrhage and an eventual necrosis of the tissue at the envenomed region are reported in several spider bites, ,. This is the first report on the isolation and characterization of a toxin from spider venom in the Indian subcontinent. Partitagin was devoid of edema, myotoxicity and lethality. Partitagin showed specificity of action on the components of ECM and degraded collagen type-IV and fibronectin but not collagen type-I. Similar intense damage was also noticed in the ECM of muscle tissue sections but with no damage caused to myocytes.
#Leitz wetzlar germany type 307 148.002 skin
Light microscopy of skin tissue sections at the site of injection revealed extensive damage of extracellular matrix (ECM) in which the basement membrane surrounding blood vessels and capillaries showing signs of extensive destruction and also loss of vessel wall integrity. Partitagin caused hemorrhage when tested in a mouse model.
The presence of Zn +2 was confirmed by atomic absorption spectrometry. EDTA, 1,10-phenanthroline and cyanide, inactivated it irreversibly while, EGTA, PMSF, leupeptin, pepstatin and aprotinin did not inhibit.
#Leitz wetzlar germany type 307 148.002 free
Partitagin was assayed using fat free casein as substrate. SDS-PAGE, reversed phase HPLC on a C 4 column, N-terminal amino acid sequencing and MALDI-TOF mass spectrometry confirmed the homogeneity. partita venom by successive chromatography on Sephadex G-100, DEAE Sephadex A-50 and Biosep DEAE columns.
In this work a hemorrhagic metalloprotease, Partitagin was purified from H. The poisonous bite by Hippasa partita, a funnel web spider from the Indian subcontinent has been demonstrated to give rise to severe dermo- and myonecrosis.
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